Rabbit anti-Lamin-A Antibody

• Catalog Number: orb1524847
• Category: Antibodies
• Description: Lamin-A Antibody
• Target: Lamin-A
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Whole IgG
• Concentration: 200 µg/ml
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Immunogen: Between 550 and 600
• UniProt ID: P02545
• Tested applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: prelamin-A/C; LDP1; LGMD1B; LMN1; LMNC; LMNL1; lam
• Note: For research use only
• NCBI: NP_733821.1

Detection of human and mouse Lamin-A by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Lamin-A antibody orb1524847 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. Lamin-A was also immunoprecipitated by rabbit anti-Lamin-A/C antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A and B).

Detection of mouse Lamin-A by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Lamin-A (Cat. No. orb1524847) used at a dilution of 1: 200 (1µg/ml). Detection: DAB