Rabbit LAG3 Recombinant Monoclonal Antibody

SKU: orb1519933

Description

LAG3 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, IP, WB
• Dilution Range: Flow-Cyt Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells. Immunocytochemistry (ICC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. Immunohistochemistry (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Immunoprecipitation (IP) 12 µl/mg lysate. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000
• Reactivity: Human
• Application Notes:
  All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: BLR027F
• Immunogen: Between 475 and C-terminus
• Target: LAG3
• Purity: Recombinant antibody was purified from cell culture supernatant
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD antigen CD223; CD223; LAG-3; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Protein FDC

Quick Database Links

Gene Symbol

LAG3

UniProt

P18627

RefSeq (Protein):NP_002277.4