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Description
Research Area
Images & Validation
−| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. For mouse tissues: 1:200 - 1:800. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Frog |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 375 and 425 |
| Target | KIAA0528 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Detection of mouse KIAA0528 by immunohistochemistry. Sample: FFPE section of mouse intestine. Antibody: Affinity purified rabbit anti-KIAA0528 used at a dilution of 1:400 (2.5µg/ml).

Detection of human KIAA0528 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-KIAA0528 used at a dilution of 1:1, 000 (1µg/ml).

Detection of human KIAA0528 by WB and IP.Samples: HeLa cells. Antibodies: Affinity purified rabbit anti-KIAA0528 antibody orb1528338 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_055617.1 |
|---|
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Protocol Information
Rabbit KIAA0528 Antibody (orb1528338)
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