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Rabbit IL-12B Recombinant Monoclonal Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IP, WB |
|---|---|
| Dilution Range | Application : WB , ApplicationDilutionRange : 1:1,000 , Application : IP , ApplicationDilutionRange : 6 µl/mg lysate |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BLR322M |
| Immunogen | A recombinant protein representing human IL-12B (residues 23-328, NP_002178.2). |
| Target | IL-12B |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, rAlbumin-Free |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Detection of human IL-12B by western blot. Samples: Whole cell lysate (50 µg) from 293F cells untransfected (-) or overexpressing IL-12A, IL-12B, IL-12A and IL-12B, or IL-23A and IL-12B. Antibody: Rabbit anti-IL-12B recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 1 second

Detection of human IL-12B by western blot. Samples: Human recombinant IL-12B protein (0.01 µg). Antibody: Rabbit anti-IL-12B recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 1 second
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_002178.2 |
|---|
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Protocol Information
Rabbit IL-12B Recombinant Monoclonal Antibody (orb2281284)
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