Rabbit Huntingtin Antibody

SKU: orb1525951

Description

Huntingtin Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1175 and 1225
• Target: Huntingtin
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HD; HD protein; huntingtin; huntington disease protein; IT15; LOMARS

Detection of human Huntingtin by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Huntingtin antibody orb1525951 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. Huntingtin was also immunoprecipitated by rabbit anti-Huntingtin antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

Quick Database Links

Gene Symbol

Huntingtin

UniProt

P42858

RefSeq (Protein):NP_002102.4