Rabbit Human C3a Antibody

SKU: orb21215

Description

C3a is a component of complement C3 produced by its activation. It has a molecular weight of 8,900. The protein is isolated and purified from pooled normal human plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Images & Validation

• Reactivity: Human
• Application Notes:
  Precipitation assays. In immunoelectrophoresis in agarose-plates use 2 μl human plasma or equivalent against 120 μl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Target: Precipitating polyclonal Rabbit antiSerum to C3a subunit of Human complement C3.
• Conjugation: Unconjugated

Storage & Handling

• Storage: The lyophilized antiserum should be stored at or below -20°C. Dilutions may be prepared by adding phosphate-buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
• Form/Appearance: Delipidated, heat inactivated, lyophilized, stable whole serum. No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Precipitating polyclonal Rabbit antiSerum to C3a subunit of Human complement C3.

UniProt

P01024