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Description
Research Area
Images & Validation
−| Tested Applications | IP |
|---|---|
| Dilution Range | WB - Not recommended. orb1526904 has not performed satisfactorily when used for WB of hSet1B in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of hSet1B.; IP - 2-10 µg/mg lysate |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 350 and 400 |
| Target | hSET1B |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Detection of human hSET1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-hSET1B antibody (orb1526904) used for IP at 6 µg per reaction. hSET1B was also immunoprecipitated by a previous lot of this antibody (orb1526904) and a second antibody against a different epitope of hSET1B.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_055863.1 |
|---|
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Rabbit hSET1B Antibody (orb1526904)
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