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Catalog Number | orb1520221 |
---|---|
Category | Antibodies |
Description | HNRNPQ/SYNCRIP Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human |
Immunogen | Between 1 and 50 |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 20-40 µl/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | HNRNPQ/SYNCRIP |
UniProt ID | O60506 |
NCBI | NP_006363.4 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | hnRNP-Q; Synaptotagmin-binding, cytoplasmic RNA-in Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human HNRNPQ/SYNCRIP by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, SW620, HCT 116, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HNRNPQ/SYNCRIP antibody (orb1520221) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Detection of human HNRNPQ/SYNCRIP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HNRNPQ/SYNCRIP antibody (orb1520221) used for IP at 2 µg per reaction. For blotting immunoprecipitated HNRNPQ/SYNCRIP, orb1520221 was used at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 1 second.