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| Catalog Number | orb1524991 |
|---|---|
| Category | Antibodies |
| Description | HIP55 Antibody |
| Target | HIP55 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Whole IgG |
| Concentration | 200 µg/ml |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Immunogen | Between 250 and 300 |
| UniProt ID | Q9UJU6 |
| Tested applications | IP, WB |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate |
| Application notes | Format: Whole IgG |
| Antibody Type | Primary Antibody |
| Storage | 2 - 8°C |
| Alternative names | SH3P7; src homology 3 domain-containing protein HI Read more... |
| Note | For research use only |
| NCBI | NP_001014436.1 |

Detection of human HIP55 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-HIP55 antibody orb1524991 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. HIP55 was also immunoprecipitated by rabbit anti-HIP55 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).
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