Rabbit HERC2 Antibody

SKU: orb1527580

Description

HERC2 Antibody

Research Area

Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 4784 and C-terminus
• Target: HERC2
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

D15F37S1; E3 ubiquitin-protein ligase HERC2; hect domain and RCC1-like domain-containing protein 2; hect domain and RLD 2; HECT-type E3 ubiquitin transferase HERC2; jdf2; MRT38; p528; probable E3 ubiquitin-protein ligase HERC2; SHEP1

Detection of mouse HERC2 by western blot. Samples: Whole cell lysate (10 µg) from CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HERC2 antibody (orb1527580) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human HERC2 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, MCF-7, Jurkat, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HERC2 antibody (orb1527580) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human HERC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HERC2 antibody (orb1527580) used for IP at 6 µg per reaction. HERC2 was also immunoprecipitated by a previous lot of this antibody (orb1527580) and a second antibody against a different epitope of HERC2.

Quick Database Links

Gene Symbol

HERC2

UniProt

O95714

RefSeq (Protein):NP_004658.3