Rabbit anti-HERC2 Antibody

• Catalog Number: orb1527580
• Category: Antibodies
• Description: HERC2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 4784 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: HERC2
• UniProt ID: O95714
• NCBI: NP_004658.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: hect domain and RCC1-like domain-containing protei
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse HERC2 by western blot. Samples: Whole cell lysate (10 µg) from CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HERC2 antibody (orb1527580) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human HERC2 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, MCF-7, Jurkat, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HERC2 antibody (orb1527580) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human HERC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HERC2 antibody (orb1527580) used for IP at 6 µg per reaction. HERC2 was also immunoprecipitated by a previous lot of this antibody (orb1527580) and a second antibody against a different epitope of HERC2.