Rabbit HCA66 Antibody

SKU: orb1526384

Description

HCA66 Antibody

Images & Validation

• Tested Applications: IHC, IP
• Dilution Range: WB - Not recommended. orb1526384 has not performed satisfactorily when used for WB of HCA66 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of HCA66.; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 547 and C-terminus
• Target: HCA66
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

C17orf40; HCA66; hepatocellular carcinoma-associated antigen 66; multiple hat domains protein; U3 small nucleolar RNA-associated protein 6 homolog; UTP6, small subunit (SSU) processome component, homolog

Detection of human HCA66 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HCA66 antibody orb1526384 used for IP at 3 µg/mg lysate.

Detection of human HCA66 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-HCA66 (Cat. No. orb1526384) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

HCA66

UniProt

Q9NYH9

RefSeq (Protein):NP_060898.2