Rabbit Hakai Antibody

SKU: orb1525681

Description

Hakai Antibody

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 441 and C-terminus
• Target: Hakai
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cas-Br-M (murine) ecotropic retroviral transforming sequence-like 1; casitas B-lineage lymphoma-transforming sequence-like protein 1; Cbl proto-oncogene like 1, E3 ubiquitin protein ligase; Cbl proto-oncogene, E3 ubiquitin protein ligase-like 1; c-Cbl-like protein 1; E3 ubiquitin-protein ligase Hakai; E-cadherin binding protein E7; HAKAI; RING finger protein 188; RING-type E3 ubiquitin transferase Hakai; RNF188

Detection of human and mouse Hakai by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Hakai antibody orb1525681 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Hakai was also immunoprecipitated by rabbit anti-Hakai antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Quick Database Links

Gene Symbol

Hakai

UniProt

Q75N03

RefSeq (Protein):NP_079090.2