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Rabbit anti-HACL1 Antibody
Catalog Number: orb1520819
| Catalog Number | orb1520819 |
|---|---|
| Category | Antibodies |
| Description | HACL1 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IP, WB |
| Reactivity | Human, Mouse |
| Immunogen | Between 528 and C-terminus |
| Antibody Type | Primary Antibody |
| Concentration | 1000 µg/ml |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 20 µl/mg lysate |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | HACL1 |
| UniProt ID | Q9UJ83 |
| NCBI | Q9UJ83.2 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Alternative names | 2-hydroxyphytanol-CoA lyase; phytanoyl-CoA hydroxy Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human and mouse HACL1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HACL1 antibody (orb1520819) used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human HACL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HACL1 antibody (orb1520819) used for IP at 2 µg per reaction.
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