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Rabbit anti-Goat IgG (H&L), ALP conjugated, min, cross-reactivity to human, mouse and Rat IgG

Rabbit anti-Goat IgG (H&L), ALP conjugated, min, cross-reactivity to human, mouse and Rat IgG

Catalog Number: orb700280

DispatchUsually dispatched within 5-10 working days
$ 630.00
Catalog Numberorb700280
CategoryAntibodies
DescriptionRabbit polyclonal antibody against Rabbit anti-Goat IgG (H&L) conjugated to AP
ClonalityPolyclonal
Species/HostRabbit
ConjugationALP
ReactivityGoat
Form/AppearanceLiquid
PurityImmunogen affinity purified rabbit IgG.
ImmunogenPurified Goat IgG, whole molecule
Tested applicationsELISA, ICC, IHC, WB
Dilution range1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Application notesNo reactivity is observed to non-immunoglobulin goat serum proteins based or IgG from human, mouse or rat based on immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
StorageNon-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
NoteFor research use only