Rabbit anti-GLI1 Recombinant Monoclonal Antibody

• Catalog Number: orb1710827
• Category: Antibodies
• Description: GLI1 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR256L
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: Between 1056 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 100 µg/ml
• Dilution range: WB: 1:1,000, IP: 20 µl/1 mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: GLI1
• UniProt ID: P08151
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: glioma-associated oncogene homolog 1 (zinc finger
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human GLI1 by western blot. Samples: Whole cell lysate (50 µg) from Hep-G2, PC-3, RMS-13, MCF-7, and TOV-112D cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GLI1 recombinant monoclonal antibody (orb1710827) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Detection of human GLI1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from RMS-13 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-GLI1 recombinant monoclonal antibody (orb1710827) used for IP at 20 µl/mg lysate.