Rabbit GCN1L1 Antibody

SKU: orb1527689

Description

GCN1L1 Antibody

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 2621 and C-terminus
• Target: GCN1L1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

eIF-2-alpha kinase activator GCN1; GCN1 (general control of amino-acid synthesis 1, yeast)-like 1; GCN1 eIF-2-alpha kinase activator homolog; GCN1 general control of amino-acid synthesis 1-like 1; GCN1L; GCN1L1; GCN1-like protein 1; general control of amino-acid synthesis 1-like protein 1; hsGCN1; peroxisome proliferator activated receptor interacting complex protein; PRIC295; translational activator GCN1

Detection of mouse and rat GCN1L1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, TCMK-1, and C6 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GCN1L1 antibody orb1527689 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human GCN1L1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, RPMI-8226, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GCN1L1 antibody orb1527689 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human GCN1L1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GCN1L1 antibody orb1527689 used for IP at 6 µg per reaction. GCN1L1 was also immunoprecipitated by a previous lot of this antibody (orb1527689).

Quick Database Links

Gene Symbol

GCN1L1

UniProt

Q92616

RefSeq (Protein):NP_006827.1