Rabbit anti-G3BP2 Antibody

• Catalog Number: orb1527341
• Category: Antibodies
• Description: G3BP2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: Between 275 and 325
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: G3BP2
• UniProt ID: Q9UN86
• NCBI: NP_036429.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: G3BP-2; GAP SH3 domain-binding protein 2; GTPase a
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human G3BP2 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, MCF-7, Jurkat, HeLa, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G3BP2 antibody (orb1527341) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human G3BP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-G3BP2 antibody (orb1527341) used for IP at 6 µg per reaction. G3BP2 was also immunoprecipitated by a previous lot of this antibody (orb1527341) and a second antibody against a different epitope of G3BP2.