Rabbit G3BP1 Antibody

SKU: orb1527355

Description

G3BP1 Antibody

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 275 and 325
• Target: G3BP1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ATP-dependent DNA helicase VIII; DNA helicase VIII; G3BP; G3BP-1; GAP binding protein; GAP SH3 domain-binding protein 1; GTPase activating protein (SH3 domain) binding protein 1; hDH VIII; HDH-VIII; ras GTPase-activating protein-binding protein 1; RasGAP-associated endoribonuclease G3BP; Ras-GTPase-activating protein SH3-domain-binding protein

Detection of human and mouse G3BP1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, MCF-7, U2OS, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G3BP1 antibody orb1527355 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of human G3BP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-G3BP1 antibody orb1527355 used for IP at 6 µg per reaction.

Quick Database Links

Gene Symbol

G3BP1

UniProt

Q13283

RefSeq (Protein):NP_005745.1