Rabbit anti-FOXP3 Recombinant Monoclonal Antibody

SKU: orb1519916

Description

FOXP3 Antibody

Images & Validation

• Tested Applications: ICC, IF, IHC, IP, WB
• Dilution range: Immunocytochemistry (ICC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. Immunohistochemistry (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Immunoprecipitation (IP) 20 µl/mg lysate. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000
• Reactivity: Human
• Application Notes:
  All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Clone No.: BLR034F
• Immunogen: Between 275 and 325
• Target: FOXP3
• Purity: Recombinant antibody was purified from cell culture supernatant
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

PIDX; scurfin; JM2; IPEX; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; FOXP3delta7; forkhead box protein P3; XPID; DIETER; AIID; immune dysregulation, polyendocrinopathy, enteropathy, X-linked

Detection of human FOXP3 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HEK293T, MJ, MOLT-4, and RPMI-8226 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody

Detection of human FOXP3 in FFPE tonsil by immunohistochemistry. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of human FOXP3 in FFPE breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB

Detection of human FOXP3 (red) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-FOXP3 recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue)

Quick Database Links

Gene Symbol

FOXP3

UniProt

Q9BZS1

RefSeq (Protein):NP_054728.2