Rabbit anti-EXOSC10 Antibody

• Catalog Number: orb1523805
• Category: Antibodies
• Description: EXOSC10 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 200 and 250
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: EXOSC10
• UniProt ID: Q01780
• NCBI: NP_001001998.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: PMSCL; PM-Scl; Rrp6p; PMSCL2; P100 polymyositis-sc
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse EXOSC10 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-EXOSC10 antibody orb1523805 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human EXOSC10 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EXOSC10 antibody orb1523805 used for IP at 6 µg/mg lysate.

Detection of human EXOSC10 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EXOSC10 (Cat. No. orb1523805) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of mouse EXOSC10 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-EXOSC10 (Cat. No. orb1523805) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB