Rabbit Endoglycan Antibody

SKU: orb1522917

Description

Endoglycan Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:5,000 - 1:2,500; IP - 2 - 10 µg/mg lysate
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 300 and 350
• Target: Endoglycan
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EG; endoglycan; PODLX2; podocalyxin-like protein 2

Detection of human Endoglycan by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Endoglycan antibody orb1522917 used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Endoglycan by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Endoglycan antibody orb1522917 used for IP at 6 µg per reaction.

Quick Database Links

Gene Symbol

Endoglycan

UniProt

Q9NZ53

RefSeq (Protein):NP_056535.1