Rabbit eIF5B/IF2 Antibody

SKU: orb1527867

Description

eIF5B/IF2 Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - Not recommended. Use rabbit anti-eIF5B/IF2 antibody A301-744A.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 450 and 500
• Target: eIF5B/IF2
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

eIF-5B; eukaryotic translation initiation factor 5B; IF2; translation initiation factor IF2; translation initiation factor IF-2

Detection of human eIF5B/IF2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eIF5B/IF2 antibody orb1527867 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. eIF5B/IF2 was immunoprecipitated successfully when using rabbit anti-eIF5B/IF2 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Quick Database Links

Gene Symbol

eIF5B/IF2

UniProt

O60841

RefSeq (Protein):NP_056988.3