Rabbit eIF4G3/eIF4GII Antibody

SKU: orb1527827

Description

eIF4G3/eIF4GII Antibody

Research Area

Cardiovascular Research, Immunology & Inflammation

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 300 and 350
• Target: eIF4G3/eIF4GII
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

eIF4G 3; eIF-4G 3; eIF-4-gamma 3; eIF-4-gamma II; eIF4GII; eukaryotic translation initiation factor 4 gamma 3

Detection of human eIF4G3/eIF4GII by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, RKO, LNCaP, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF4G3/eIF4GII antibody orb1527827 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human eIF4G3/eIF4GII by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF4G3/eIF4GII antibody orb1527827 used for IP at 6 µg per reaction.

Quick Database Links

Gene Symbol

eIF4G3/eIF4GII

UniProt

O43432

RefSeq (Protein):NP_003751.2