Rabbit eIF3E Antibody

SKU: orb1525651

Description

eIF3E Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 50 and 100
• Target: eIF3E
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

eIF-3 p48; eIF3e; eIF3-p46; EIF3-P48; EIF3S6; eukaryotic translation initiation factor 3 subunit 6; eukaryotic translation initiation factor 3 subunit E; eukaryotic translation initiation factor 3 subunit E isoform 2 transcript; eukaryotic translation initiation factor 3, subunit 6 (48kD); eukaryotic translation initiation factor 3, subunit 6 48kDa; INT6; mammary tumor-associated protein INT6; murine mammary tumor integration site 6 (oncogene homolog); viral integration site protein INT-6 homolog

Detection of human and mouse eIF3E by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eIF3E antibody orb1525651 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. eIF3E was also immunoprecipitated by rabbit anti-eIF3E antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).

Detection of human eIF3E by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-eIF3E (Cat. No. orb1525651) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

eIF3E

UniProt

P60228

RefSeq (Protein):NP_001559.1