Rabbit anti-eIF3B/EIF3S9 Antibody

SKU: orb1527841

Description

eIF3B/EIF3S9 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 100 and 150
• Target: eIF3B/EIF3S9

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD); prt1 homolog; PRT1; eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa; eukaryotic translation initiation factor 3 subunit B; eukaryotic translation initiation factor 3 subunit 9; EIF3S9; EIF3-P110; EIF3-ETA; eIF3 p110; eIF-3-eta; hPrt1; eIF3b; eIF3 p116; EIF3-P116

Detection of human eIF3B by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF3B antibody orb1527841 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human eIF3B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF3B antibody orb1527841 used for IP at 3 µg per reaction.

Detection of human EIF3S9/eIF3B by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-EIF3S9/eIF3B (Cat. No. orb1527841) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

eIF3B/EIF3S9

UniProt

P55884

RefSeq (Protein):NP_003742.2