Rabbit eIF3A/eIF3S10 Antibody

SKU: orb1527405

Description

eIF3A/eIF3S10 Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 or Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 875 and 925
• Target: eIF3A/eIF3S10
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

centrosomin homolog; cytoplasmic protein p167; EIF3; eIF3 p167; eIF3 p180; eIF3 p185; EIF3, p180 subunit; eIF3a; eIF3-p170; EIF3S10; eIF-3-theta; eIF3-theta; eukaryotic translation initiation factor 3 subunit 10; eukaryotic translation initiation factor 3 subunit A; eukaryotic translation initiation factor 3, subunit 10 (theta, 150/170kD); eukaryotic translation initiation factor 3, subunit 10 (theta, 170kD); eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa; eukaryotic translation initiation factor 3, subunit 10, 170kD; P167; p180; p185; TIF32

Detection of human and mouse eIF3A/eIF3S10 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF3A/eIF3S10 antibody orb1527405 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human eIF3A/eIF3S10 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF3A/eIF3S10 antibody orb1527405 used for IP at 6 µg per reaction.

Detection of mouse eIF3A/EIF3S10 by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-eIF3A/EIF3S10 (Cat. No. orb1527405) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Detection of human eIF3A/EIF3S10 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-eIF3A/EIF3S10 (Cat. No. orb1527405) used at a dilution of 1: 1, 000 (0.2µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

eIF3A/eIF3S10

UniProt

Q14152

RefSeq (Protein):NP_003741.1