Rabbit eIF2B delta/EIF2B4 Antibody

SKU: orb1525657

Description

eIF2B delta/EIF2B4 Antibody

Research Area

Metabolism Research, Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 50 and 100
• Target: eIF2B delta/EIF2B4
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EIF2B; EIF-2B; eIF-2B GDP-GTP exchange factor subunit delta; EIF2Bdelta; eukaryotic translation initiation factor 2B subunit 4 delta; eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa; translation initiation factor eIF-2b delta subunit; translation initiation factor eIF-2B subunit delta

Detection of human eIF2B delta/EIF2B4 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eIF2B delta/EIF2B4 antibody orb1525657 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. eIF2B delta/EIF2B4 was also immunoprecipitated by rabbit anti-eIF2B delta/EIF2B4 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Detection of human eIF2B delta/EIF2B4 by immunohistochemistry. Samples: FFPE sections of human colon carcinoma and breast carcinoma. Antibody: Affinity purified rabbit anti-eIF2B delta/EIF2B4 (Cat. No. orb1525657) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

eIF2B delta/EIF2B4

UniProt

Q9UI10

RefSeq (Protein):NP_056451.3