Rabbit anti-EDD1 Antibody

• Catalog Number: orb1524641
• Category: Antibodies
• Description: EDD1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 625 and 675
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: EDD1
• UniProt ID: O95071
• NCBI: NP_056986.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: HECT-type E3 ubiquitin transferase UBR5; DD5; prog
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human EDD1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-EDD1 antibody orb1524641 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. EDD1 was also immunoprecipitated by rabbit anti-EDD1 antibodies, which recognizes a downstream epitope, and orb1529971, which recognizes a similar epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

Detection of human EDD1 by immunohistochemistry.Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-EDD1 (Cat. No. orb1524641) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: hematoxylin (blue).