Rabbit anti-ECS (DYKDDDDK) Tag Recombinant Monoclonal Antibody

SKU: orb1530835

Description

ECS (DYKDDDDK) Tag Antibody

Images & Validation

• Tested Applications: ELISA, ICC, IP, WB
• Dilution range: WB - 1:1000; IP - 20 µl/mg lysate; ICC - 1:1000 to 1:5000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; ELISA - 1:1,000 - 1:75,000; for coating plates 1:100 - 1:500
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Recombinant
• Clone No.: BLRE00G
• Immunogen: Enterokinase Cleavage Site (DYKDDDDK)
• Target: ECS (DYKDDDDK) Tag
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 100 µg/ml
• Disclaimer: For research use only

Detection of ECS (DYKDDDDK)-tagged protein by WB from non-transfected HEK293 cells, HEK293 transfected with Met-ECS(DYKDDDDK)-IDO1, IDO1-ECS(DYKDDDDK)-HIS (Internal Tag), IDO1-HIS-ECS(DYKDDDDK) (C-terminal Tag), and SUP-M2 cells, at 1:1000.

Detection of ECS (DYKDDDDK) Tag by western blot of immunoprecipitates from HEK293 transfected with IDO1-HIS-ECS(DYKDDDDK) (C-terminal Tag) lysate.Antibody: Rabbit anti-ECS (DYKDDDDK) Tag recombinant monoclonal antibody.

Detection of ECS (DYKDDDDK) Tag by western blot of immunoprecipitates from HEK293 transfected with Met-ECS(DYKDDDDK)-IDO1 lysate.Antibody: Rabbit anti-ECS (DYKDDDDK) Tag recombinant monoclonal antibody.

Detection of ECS (DYKDDDDK) Tag in FFPE HEK293 cells expressing ECS (DYKDDDDK)-tagged nuclear protein by ICC.Antibody: Rabbit anti-ECS (DYKDDDDK) Tag recombinant monoclonal antibody.

Detection of ECS (DYKDDDDK) Tag in FFPE HEK293 cells expressing an ECS (DYKDDDDK)-tagged cytoplasmic protein by ICC.Antibody: Rabbit anti-ECS (DYKDDDDK) Tag recombinant monoclonal antibody.

Quick Database Links

Gene Symbol

ECS (DYKDDDDK) Tag