Rabbit E-Selectin/CD62E Recombinant Monoclonal Antibody

SKU: orb1806428

Description

Rabbit E-Selectin/CD62E Recombinant Monoclonal Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation

Images & Validation

• Tested Applications: FC, ICC, IHC, IP, WB
• Dilution range: Application : Flow-Cyt , ApplicationDilutionRange : Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells. , Application : ICC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. , Application : IHC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. , Application : IP , ApplicationDilutionRange : 6 µl/mg lysate , Application : WB , ApplicationDilutionRange : 1:1000
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: BLR295L
• Immunogen: Residues 22-555 (ECD)
• Target: E-Selectin/CD62E
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

CD antigen CD62E;CD62 antigen-like family member E;CD62E;ELAM;ELAM1;ELAM-1;endothelial adhesion molecule 1;endothelial leukocyte adhesion molecule 1;ESEL;E-selectin;LECAM2;leukocyte endothelial cell adhesion molecule 2;Leukocyte-endothelial cell adhesion molecule 2

Detection of human E-Selectin/CD62E by western blot. Samples: Whole cell lysate (50 µg) from HUVEC-C cells untreated (-) or treated (+) with 10ng/mL TNFalpha. Antibody: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (orb1806428) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human E-Selectin/CD62E by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (orb1806428). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human E-Selectin/CD62E (shaded) in TNF-alpha treated HUVEC cells by flow cytometry. Antibody: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (orb1806428) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human E-Selectin/CD62E by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HUVEC-C cells treated with 10ng/mL TNFalpha. Antibodies: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (orb1806428) used for IP at 6 µl/mg lysate.

Detection of human E-Selectin/CD62E by immunocytochemistry. Sample: FFPE section of HUVEC-C cells treated with TNF-alpha (left) and mock treated HUVEC-C cells (right). Antibody: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (orb1806428). Secondary: HRP-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

E-Selectin/CD62E

UniProt

P16581

RefSeq (Protein):NP_000441.2