Rabbit anti-E-Selectin/CD62E Recombinant Monoclonal Antibody

• Catalog Number: orb1806428
• Category: Antibodies
• Description: Rabbit anti-E-Selectin/CD62E Recombinant Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR295L
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human
• Immunogen: Residues 22-555 (ECD)
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB: 1:1000, IP: 6 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: E-Selectin/CD62E
• UniProt ID: P16581
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: endothelial adhesion molecule 1; endothelial leuko
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human E-Selectin/CD62E by western blot. Samples: Whole cell lysate (50 µg) from HUVEC-C cells untreated (-) or treated (+) with 10ng/mL TNFalpha. Antibody: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (orb1806428) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human E-Selectin/CD62E by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (orb1806428). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human E-Selectin/CD62E (shaded) in TNF-alpha treated HUVEC cells by flow cytometry. Antibody: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (orb1806428) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human E-Selectin/CD62E by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HUVEC-C cells treated with 10ng/mL TNFalpha. Antibodies: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (orb1806428) used for IP at 6 µl/mg lysate.

Detection of human E-Selectin/CD62E by immunocytochemistry. Sample: FFPE section of HUVEC-C cells treated with TNF-alpha (left) and mock treated HUVEC-C cells (right). Antibody: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (orb1806428). Secondary: HRP-conjugated goat anti-rabbit IgG.