Rabbit DYNC1I2 Antibody

SKU: orb1522835

Description

DYNC1I2 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 588 and C-terminus
• Target: DYNC1I2
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

cytoplasmic dynein 1 intermediate chain 2; Cytoplasmic dynein intermediate chain 2; DH IC-2; DIC74; DNCI2; dynein intermediate chain 2, cytosolic; dynein, cytoplasmic, intermediate polypeptide 2; IC2; testis tissue sperm-binding protein Li 66n

Detection of human and mouse DYNC1I2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DYNC1I2 antibody orb1522835 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human DYNC1I2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DYNC1I2 antibody orb1522835 used for IP at 6 µg per reaction.

Detection of human DYNC1I2 by immunohistochemistry. Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti-DYNC1I2 used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of mouse DYNC1I2 by immunohistochemistry. Samples: FFPE serial sections of mouse teratoma. Antibody: Affinity purified rabbit anti-DYNC1I2 used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

DYNC1I2

UniProt

Q13409

RefSeq (Protein):NP_001369.1