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Catalog Number | orb1523260 |
---|---|
Category | Antibodies |
Description | DPY30 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP |
Reactivity | Human, Mouse |
Immunogen | Between 1 and 50 |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - Not recommended. orb1523260 has not performed satisfactorily when used for WB of DPY30 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of DPY30.; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | DPY30 |
UniProt ID | Q9C005 |
NCBI | NP_115963.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | Cps25; dpy-30 homolog; dpy-30L; dpy-30-like protei Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human DPY30 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-DPY30 antibody orb1523260 used for IP at 6 µg per reaction.
Detection of mouse DPY30 by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-DPY30 (Cat. No. orb1523260) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Detection of human DPY30 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DPY30 (Cat. No. orb1523260) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).