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Rabbit DPY30 Antibody
Description
Images & Validation
−| Tested Applications | IHC, IP |
|---|---|
| Dilution Range | WB - Not recommended. orb1523260 has not performed satisfactorily when used for WB of DPY30 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of DPY30.; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 1 and 50 |
| Target | DPY30 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Detection of human DPY30 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-DPY30 antibody orb1523260 used for IP at 6 µg per reaction.

Detection of mouse DPY30 by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-DPY30 (Cat. No. orb1523260) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human DPY30 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DPY30 (Cat. No. orb1523260) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_115963.1 |
|---|
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Protocol Information
Rabbit DPY30 Antibody (orb1523260)
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