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Catalog Number | orb1530270 |
---|---|
Category | Antibodies |
Description | DHX15 Antibody |
Target | DHX15 |
Clonality | Polyclonal |
Species/Host | Rabbit |
Conjugation | Unconjugated |
Reactivity | Human |
Form/Appearance | Whole IgG |
Concentration | 1000 µg/ml |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Immunogen | Between 350 and 400 |
UniProt ID | O43143 |
Tested applications | IHC, WB |
Dilution range | WB - 1:5,000 - 1:15,000; IP - Not recommended. A300-390A has performed well and is suggested for IP.; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Application notes | Format: Whole IgG |
Antibody Type | Primary Antibody |
Storage | 2 - 8°C |
Alternative names | PRP43; RNA helicase 2; putative pre-mRNA-splicing Read more... |
Note | For research use only |
NCBI | NP_001349.2 |
Expiration Date | 12 months from date of receipt. |
Detection of human DHX15 by western blot and immunoprecipitation.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-DHX15 antibody (orb1530270) used for WB and IP.
Detection of human DHX15 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DHX15 used at a dilution of 1:1, 000 (1µg/ml).