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Rabbit anti-DDX42 Antibody
Catalog Number: orb1524985
| Catalog Number | orb1524985 |
|---|---|
| Category | Antibodies |
| Description | DDX42 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IP, WB |
| Reactivity | Human, Mouse |
| Immunogen | Between 888 and C-terminus |
| Antibody Type | Primary Antibody |
| Concentration | 1000 µg/ml |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | DDX42 |
| UniProt ID | Q86XP3 |
| NCBI | NP_031398.2 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Alternative names | SF3b DEAD box protein; splicing factor 3B-associat Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human and mouse DDX42 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-DDX42 antibody orb1524985 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. DDX42 was also immunoprecipitated by rabbit anti-DDX42 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
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