Rabbit DCTN2 Antibody

SKU: orb1524743

Description

DCTN2 Antibody

Research Area

Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Frog, Gallus, Rat, Xenopus
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1 and 50
• Target: DCTN2
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

50 kDa dynein-associated polypeptide; DCTN50; DCTN-50; dynactin 2 (p50); dynactin complex 50 kDa subunit; dynactin subunit 2; DYNAMITIN; epididymis secretory protein Li 77; epididymis secretory sperm binding protein; HEL-S-77; p50 dynamitin; RBP50

Detection of human and mouse DCTN2 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from Jurkat (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , HeLa (H; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-DCTN2 antibody orb1524743 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. DCTN2 was also immunoprecipitated by rabbit anti-DCTN2 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Quick Database Links

Gene Symbol

DCTN2

UniProt

Q13561

RefSeq (Protein):NP_006391.1