Rabbit DCTN2 Antibody

SKU: orb1524741

Description

DCTN2 Antibody

Research Area

Immunology & Inflammation, Neuroscience

Images & Validation

−

Item 1 of 2

Tested Applications	IHC, IP, WB
Dilution Range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 351 and C-terminus
Target	DCTN2
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

−

Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

50 kDa dynein-associated polypeptide; DCTN50; DCTN-50; dynactin 2 (p50); dynactin complex 50 kDa subunit; dynactin subunit 2; DYNAMITIN; epididymis secretory protein Li 77; epididymis secretory sperm binding protein; HEL-S-77; p50 dynamitin; RBP50

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Detection of human DCTN2 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified rabbit anti-DCTN2 antibody orb1524741 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. DCTN2 was also immunoprecipitated by rabbit anti-DCTN2 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).

Detection of human DCTN2 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DCTN2 (Cat. No. orb1524741) used at a dilution of 1: 200 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Quick Database Links

Gene Symbol

DCTN2

UniProt

Q13561

RefSeq (Protein):NP_006391.1

UniProt Details

−

No UniProt data available

NCBI Reference Sequences

−

Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_006391.1

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

Immunoprecipitation

View Protocol

Find More Protocols