Rabbit anti-Cyclin C Antibody

• Catalog Number: orb1527425
• Category: Antibodies
• Description: Cyclin C Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 233 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Cyclin C
• UniProt ID: P24863
• NCBI: NP_005181.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: SRB11 homolog; SRB11; CycC; hSRB11; cyclin-C
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Cyclin C by western blot. Samples: Whole cell lysate (10 µg) from RKO, Jurkat, HEK293T, HeLa, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin C antibody (orb1527425) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Cyclin C by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin C antibody (orb1527425) used for IP at 6 µg per reaction.

Detection of human Cyclin C by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cyclin C (orb1527425) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB