Rabbit Cul2 Antibody

SKU: orb1526554

Description

Cul2 Antibody

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: IHC, IP
• Dilution Range: WB - Not recommended. Use rabbit anti-Cul2 antibody A302-476A.; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 150 and 200
• Target: Cul2
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CUL-2; cullin-2; testis secretory sperm-binding protein Li 238E

Detection of human Cul2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Cul2 antibody orb1526554 used for IP at 3 µg/mg lysate.

Detection of human and mouse Cul2 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-Cul2 (Cat. No. orb1526554) used at a dilution of 1: 5, 000 (0.2µg/ml) and 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

Cul2

UniProt

Q13617

RefSeq (Protein):NP_003582.2