Rabbit Cortactin Antibody

SKU: orb1526326

Description

Cortactin Antibody

Research Area

Cancer Biology, Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 450 and 500
• Target: Cortactin
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

1110020L01Rik; amplaxin; EMS1; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate)); epididymis secretory sperm binding protein; oncogene EMS1; src substrate cortactin

Detection of human Cortactin by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Cortactin antibody orb1526326 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. Cortactin was also immunoprecipitated by rabbit anti-Cortactin antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).

Detection of human Cortactin by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Cortactin (Cat. No. orb1526326) used at a dilution of 1: 1, 000 (0.2µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

Cortactin

UniProt

Q14247

RefSeq (Protein):NP_612632.1