Rabbit COPB2 Antibody

SKU: orb1522849

Description

COPB2 Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 800 and 850
• Target: COPB2
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

beta'-coat protein; beta'-COP; betaprime-COP; coatomer binding complex, beta prime subunit; coatomer protein complex subunit beta prime; coatomer protein complex, subunit beta 2 (beta prime); coatomer subunit beta'; MCPH19; p102

Detection of human and mouse COPB2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COPB2 antibody orb1522849 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human COPB2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COPB2 antibody orb1522849 used for IP at 6 µg per reaction.

Detection of human COPB2 by immunohistochemistry. Samples: FFPE serial sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-COPB2 used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of mouse COPB2 by immunohistochemistry. Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-COPB2 used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

COPB2

UniProt

P35606

RefSeq (Protein):NP_004757.1