Rabbit anti-CNOT2 Antibody

SKU: orb1526410

Description

CNOT2 Antibody

Images & Validation

• Tested Applications: IHC, IP
• Dilution range: WB - Not recommended. orb1526410 has not performed satisfactorily when used for WB of CNOT2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of CNOT2.; IP - 5 - 15 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 490 and C-terminus
• Target: CNOT2

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

CCR4-NOT transcription complex subunit 2; NOT2H; NOT2; negative regulator of transcription 2; CDC36; CCR4-associated factor 2; HSPC131

Detection of human CNOT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CNOT2 antibody orb1526410 used for IP at 10 µg/mg lysate.

Detection of human CNOT2 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CNOT (Cat. No. orb1526410) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

CNOT2

UniProt

Q9NZN8

RefSeq (Protein):NP_055330.1