Rabbit CLIP170 Antibody

SKU: orb1525002

Description

CLIP170 Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1388 and C-terminus
• Target: CLIP170
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CAP-Gly domain-containing linker protein 1; CLIP; CLIP170; CLIP-170; CYLN1; cytoplasmic linker protein 1; cytoplasmic linker protein 170 alpha-2; cytoplasmic linker protein CLIP-170; Reed-Sternberg intermediate filament-associated protein; Restin; restin (Reed-Steinberg cell-expressed intermediate filament-associated protein); RSN

Detection of human CLIP170 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CLIP170 antibody orb1525002 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. CLIP170 was also immunoprecipitated by rabbit anti-CLIP170 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Detection of human CLIP170 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CLIP170 (Cat. No. orb1525002) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Quick Database Links

Gene Symbol

CLIP170

UniProt

P30622

RefSeq (Protein):NP_937883.1