Rabbit Chicken IgY (H&L) Antibody (Alkaline Phosphatase)

SKU: orb1236952

Description

Rabbit Chicken IgY (H&L) Antibody (Alkaline Phosphatase)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Reactivity: Gallus
• Application Notes:
  Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 mg of Chicken IgY in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 is suggested for this product. (Optimal dilution should be determined by user).

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Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Isotype: IgY
• Immunogen: Chicken IgY - whole molecule
• Purification: Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgY coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Chicken IgY and Chicken Serum.
• Conjugation: AP

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0; 0.1% (w/v) Sodium Azide; 10 mg/mL Bovine Serum Albumin (rAlbumin)
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only