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Catalog Number | orb1528802 |
---|---|
Category | Antibodies |
Description | CHD8 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 2531 and C-terminus |
Antibody Type | Primary Antibody |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 to 1:1000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | CHD8 |
UniProt ID | Q9HCK8 |
NCBI | NP_065971.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | CHD-8; HELSNF1; helicase with SNF2 domain 1; chrom Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human and mouse CHD8 by western blot.Samples: Whole cell lysate from HEK293T, K-562, MCF-7, and NIH 3T3 cells. Antibody: Affinity purified Rabbit anti-CHD8 antibody orb1528802 at 0.1 µg/ml.
Detection of human CHD8 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHD8 used at a dilution of 1:1000 (0.2µg/ml).
Detection of mouse CHD8 by immunohistochemistry. Sample: FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified rabbit anti-CHD8 used at a dilution of 1:1000 (0.2µg/ml).
Detection of human CHD8 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Rabbit anti-CHD8 antibody.