Rabbit CHCHD4 Antibody

SKU: orb1520395

Description

CHCHD4 Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:10,000 - 1:25,000; IP - 50-100 µl/mg lysate
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 92 and C-terminus
• Target: CHCHD4
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

coiled-coil-helix-coiled-coil-helix domain-containing protein 4; MIA40; mitochondrial intermembrane space import and assembly protein 40; TIMM40; translocase of inner mitochondrial membrane 40 homolog

Detection of human CHCHD4 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHCHD4 antibody (orb1520395) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human CHCHD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CHCHD4 antibody (orb1520395) used for IP at 1 µg per reaction.

Quick Database Links

Gene Symbol

CHCHD4

UniProt

Q8N4Q1

RefSeq (Protein):NP_653237.1