Rabbit CEP290 Antibody

SKU: orb1528009

Description

CEP290 Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ICC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; ICC-IF - 1:250 - 1:1,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 2429 and C-terminus
• Target: CEP290
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

3H11Ag; Bardet-Biedl syndrome 14 protein; BBS14; cancer/testis antigen 87; centrosomal protein 290kDa; centrosomal protein of 290 kDa; Cep290; CT87; CTCL tumor antigen se2-2; JBTS5; LCA10; Meckel syndrome, type 4; MKS4; monoclonal antibody 3H11 antigen; Nephrocystin-6; nephrocytsin-6; NPHP6; POC3; POC3 centriolar protein homolog; prostate cancer antigen T21; rd16; SLSN6; tumor antigen se2-2

Detection of human CEP290 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CEP290 antibody orb1528009 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. CEP290 was also immunoprecipitated by rabbit anti-CEP290 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 second (B).

Detection of human CEP290 by immunocytochemistry.Sample: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-CEP290 (Cat. No. orb1528009) used at a dilution of 1: 500 (2 µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 used at a dilution of 1: 100.

Quick Database Links

Gene Symbol

CEP290

UniProt

O15078

RefSeq (Protein):NP_079390.3