Rabbit CD44 Antibody

SKU: orb1524021

Description

CD44 Antibody

Research Area

Cancer Biology, Immunology & Inflammation, Metabolism Research

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Equine, Golden Hamster
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 692 and C-terminus
• Target: CD44
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD antigen CD44; CD44 antigen; CDW44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCELL; hematopoietic cell E- and L-selectin ligand; heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; hyaluronate receptor; IN; Indian blood group antigen; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; PGP-1; PGP-I; phagocytic glycoprotein 1; Phagocytic glycoprotein I; soluble CD44

Detection of mouse CD44 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CD44 antibody orb1524021 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human CD44 by western blot. Samples: Whole cell lysate (10 µg) from A-549, HEK293T, HeLa, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CD44 antibody orb1524021 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human CD44 by immunohistochemistry.Sample: FFPE section of human melanoma.Antibody: Affinity purified rabbit anti-CD44 (Cat. No. orb1524021) used at a dilution of 1: 5, 000 (0.2µg/ml). Substrate: DAB

Detection of human CD44 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 mg for IP; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CD44 antibody orb1524021 used for IP at 2 µg per reaction.

Detection of human CD44 by immunohistochemistry.Sample: FFPE section of human head and neck squamous cell carcinoma.Antibody: Affinity purified rabbit anti-CD44 (Cat. No. orb1524021) used at a dilution of 1: 5, 000 (0.2µg/ml). Substrate: DAB

Quick Database Links

Gene Symbol

CD44

UniProt

P16070

RefSeq (Protein):NP_000601.3