Rabbit CD137 Recombinant Monoclonal Antibody

SKU: orb1519873

Description

CD137 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, ICC, IHC, IP, WB
• Dilution Range: WB - 1:1000; IP - 20 µl/1 mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: BLR051F
• Immunogen: Residues 24-189 (ECD)
• Target: CD137
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

4-1BB; 4-1BB ligand receptor; CD antigen CD137; CD137; CD137 antigen; CDw137; homolog of mouse 4-1BB; ILA; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; receptor protein 4-1BB; T cell antigen ILA; T-cell antigen 4-1BB homolog; T-cell antigen ILA; tumor necrosis factor receptor superfamily member 9

Detection of human CD137 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, HDLM-2, My-La CD4+, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD137 recombinant monoclonal antibody (orb1519873) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human CD137 in FFPE tonsil by IHC. Antibody: Rabbit anti-CD137 recombinant monoclonal antibody (orb1519873). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of human CD137 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD137 recombinant monoclonal antibody (orb1519873) used for IP at 6 µl/mg lysate. CD137 was also immunoprecipitated by a previous lot of this antibody (orb1519873). For blotting immunoprecipitated CD137, orb1519873 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human CD137 in FFPE HDLM-2 by ICC. Antibody: Rabbit anti-CD137 recombinant monoclonal antibody (orb1519873). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Quick Database Links

Gene Symbol

CD137

UniProt

Q07011

RefSeq (Protein):NP_001552.2