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Rabbit anti-Cbl-b Antibody
Catalog Number: orb1525791
| Catalog Number | orb1525791 |
|---|---|
| Category | Antibodies |
| Description | Cbl-b Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IP, WB |
| Reactivity | Human |
| Immunogen | Between 932 and C-terminus |
| Antibody Type | Primary Antibody |
| Concentration | 1000 µg/ml |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | Cbl-b |
| UniProt ID | Q13191 |
| NCBI | NP_733762.2 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Alternative names | RING-type E3 ubiquitin transferase CBL-B; signal t Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human Cbl-b by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Cbl-b antibody orb1525791 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Cbl-b was also immunoprecipitated by rabbit anti-Cbl-b antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).
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