Rabbit CaMKK alpha Antibody

SKU: orb1526212

Description

CaMKK alpha Antibody

Research Area

Cardiovascular Research, Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 455 and C-terminus
• Target: CaMKK alpha
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

calcium/calmodulin-dependent protein kinase kinase 1; calcium/calmodulin-dependent protein kinase kinase 1, alpha; calcium/calmodulin-dependent protein kinase kinase alpha; caM-kinase IV kinase; caM-kinase kinase 1; caM-kinase kinase alpha; caMKK 1; caM-KK 1; CaMKK alpha; caM-KK alpha; CAMKK alpha protein; CAMKKA

Detection of human CaMKK alpha by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CaMKK alpha antibody orb1526212 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. CaMKK alpha was also immunoprecipitated by rabbit anti-CaMKK alpha antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Detection of human CaMKK alpha by immunohistochemistry.Sample: FFPE section of human lung carcinoma (left) and human prostate carcinoma (right). Antibody: Affinity purified rabbit anti-CaMKK alpha (Cat. No. orb1526212) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

CaMKK alpha

UniProt

Q8N5S9

RefSeq (Protein):NP_115670.1